| Description | This product is a murine hybridoma IgM antibody produced by immunizing Balb/c mice with a glycoconjugate vaccine containing soluble Candida albicans β-Dextran preparation (GGZym). mAb-IgM was shown to bind to the cell wall of fungal pathogens (C. albicans, Aspergillus fumigatus, and Cryptococcus neoformans), but did not confer protection in experimental models of C. albicans infection. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Candida albicans and Cryptococcus neoformans |
| Immunogen | A glycoconjugate vaccine containing soluble Candida albicans β-Dextran preparation (GGZym) |
| Isotype | IgM |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | 1,3-Beta-glucan |
| Alternative Name | 1,3-Beta-glucan synthase; Glucan_synthase |
| Research Area | Microbiology |
Figure 1 Anti-1,3-Beta-glucan Neutralizing Antibody (V3S-0723-FY118) in SDS-PAGE.
SDS-PAGE analysis of V3S-0723-FY118 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 70 kDa and 23 kDa, respectively.
Figure 1 Anti-1,3-Beta-glucan Neutralizing Antibody (V3S-0723-FY118) in SDS-PAGE.
Figure 2 Anti-1,3-Beta-glucan Neutralizing Antibody (V3S-0723-FY118) in SEC-HPLC.
The purity of V3S-0723-FY118 was 99.83% as determined by SEC-HPLC.
Figure 2 Anti-1,3-Beta-glucan Neutralizing Antibody (V3S-0723-FY118) in SEC-HPLC.
