This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for Vaccinia virus A33, Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It can be used for A33 detection in Enzyme-Linked Immunosorbent Assay, Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
The affinity is in nanomolar range, about 0.46 nM, determined by surface plasmon resonance.
HEK293F or CHO Cell line
Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
>95%, determined by SDS-PAGE and SEC-HPLC
<1 EU/mg, determined by LAL method
Purified with Protein A or G affinity chromatography
0.2 μM filtered
PBS, pH 7.4
Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.