| Sub Cat | Host Species | Isotype | |
|---|---|---|---|
| V3S-1124-YC1664 | Human | IgG4 kappa | |
| V3S-0522-YC8 | Mouse | IgG1 kappa |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Atypical chemokine receptor 1 (Duffy blood group). The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | CHO cells expressing the recombinant Duffy glycoprotein |
| Epitope | The epitope is FEDVW. |
| Affinity | KD = 1.1 nM |
| Isotype | IgG4, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; Block |
| Application Notes | The antibody is recommended for detection of ACKR1 by ELISA, FC, Block assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ACKR1 |
| Alternative Name | Atypical Chemokine Receptor 1 (Duffy Blood Group); Duffy Blood Group, Atypical Chemokine Receptor; Duffy Blood Group, Chemokine Receptor; Plasmodium Vivax Receptor; Fy Glycoprotein; Glycoprotein D; DARC; GpFy; GPD; FY; Duffy Blood Group Chemokine Receptor; Duffy Antigen/Chemokine Receptor; Atypical Chemokine Receptor 1; Duffy Blood Group Antigen; Duffy Blood Group; CD234 Antigen; CCBP1; CD234; WBCQ1; Dfy |
| Gene ID | 2532 |
| UniProt | Q16570 |
| Research Area | Cardiovascular |
