Anti-ACT Neutralizing Antibody (V3S-1022-YC500) (CAT#: V3S-1022-YC500)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for Adenylate cyclase toxin, ACT is a 177kDa protein toxin, consisting of four sequential domains: the catalytically active N-terminal adenylate cyclase (CAT) domain, the central hydrophobic (HP) domain, modification (Mod) region carrying two acylation sites K860 and K983, the C-terminal repeat-in-toxin (RTX). It can be used for ACT detection in Enzyme-Linked Immunosorbent Assay, Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; Neut

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ACT
Alternative Name Adenylate cyclase toxin; ACT
Research Area Cell Biology
Related Disease Bordetella
For research use only, not directly for clinical use.
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