Description | The antibody is a mouse monoclonal antibody that specifically targets the human ACT protein. It has a high binding affinity of 1.2 nM for RTX751 and effectively inhibits the interaction between ACT and its receptor, with an IC₅₀ of 6.0 nM. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Bordetella strains |
Immunogen | The antibodies were obtained from mice immunized with ACT or RTX. |
IC50 | 6 nM for ACT-receptor |
Affinity | KD = 1.2 nM |
Isotype | Mouse IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | Inhib; ELISA; Neut; FC |
Application Notes | FC: 60 μg/mL |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ACT |
Alternative Name | Bordetella adenylate cyclase toxin (ACT); ACT |
Research Area | Microbiology |
Related Disease | Bordetella |