| Description | The antibody is a mouse monoclonal antibody that specifically targets the human ACT protein. It has a high binding affinity of 1.2 nM for RTX751 and effectively inhibits the interaction between ACT and its receptor, with an IC₅₀ of 6.0 nM. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Bordetella strains |
| Immunogen | The antibodies were obtained from mice immunized with ACT or RTX. |
| IC50 | 6 nM for ACT-receptor |
| Affinity | KD = 1.2 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | Inhib; ELISA; Neut; FC |
| Application Notes | FC: 60 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ACT |
| Alternative Name | Bordetella adenylate cyclase toxin (ACT); ACT |
| Research Area | Microbiology |
| Related Disease | Bordetella |