Anti-ANGPT2 (aa 117-496) Neutralizing Antibody (V3S-0522-YC2893) (CAT#: V3S-0522-YC2893)

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Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Angiopoietin 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen Recombinant human Ang-2
Epitope The epitope is in residues 117-496, most likely in the fibrinogen binding domain.
Affinity KD = 5.6 nM, determined by surface plasmon resonance.
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application ELISA; WB; Block; FuncS
Application Notes The antibody is recommended for detection of ANGPT2 by ELISA, WB, Block, FuncS assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target ANGPT2
Alternative Name Angiopoietin 2; ANG-2; Angiopoietin-2B; Angiopoietin-2a; Tie2-Ligand; AGPT2; ANG2
Gene ID 285
UniProt O15123
Research Area Cardiovascular
Related Disease Ang-2 associated disease
For research use only, not directly for clinical use.
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