| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize ANGPTL4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Mouse |
| Immunogen | Mouse ANGPTL4 |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FuncS |
| Application Notes | The antibody is recommended for detection of ANGPTL4 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ANGPTL4 |
| Alternative Name | ANGPTL4; angiopoietin-like 4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL |
| Research Area | Cell Biology |
Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC792 in reduced and non-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 150-200 kDa.
Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SDS-PAGE
Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SEC-HPLC
The purity of V3S-0622-YC792 was greater than 97% as determined by SEC-HPLC.
Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SEC-HPLC
Figure 3 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in WB
Western blot analysis of V3S-0622-YC792 was performed by loading Recombinant Mouse ANGPTL4 Protein.
Antigen: Recombinant Mouse ANGPTL4 Protein
V3S-0622-YC792 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 3 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in WB
Figure 4 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in ELISA
ELISA analysis of V3S-0622-YC792 was performed by coating with Recombinant Mouse ANGPTL4 Protein.
Figure 4 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in ELISA
