Anti-ANGPTL4 Neutralizing Antibody (V3S-0622-YC792)

Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SDS-PAGE
Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SEC-HPLC
Figure 3 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in WB
Figure 4 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in ELISA

Datasheet

MSDS

COA

Summary

Description	This product is a monoclonal antibody derived from mouse, which can specifically recognize ANGPTL4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality	Monoclonal
Host Species	Mouse
Target Species	Mouse
Immunogen	Mouse ANGPTL4
Isotype	IgG2a

Tested Data

SDS-PAGE

Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC792 in reduced and non-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 150-200 kDa.

Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SEC-HPLC

The purity of V3S-0622-YC792 was greater than 97% as determined by SEC-HPLC.

Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in SEC-HPLC

Figure 3 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in WB

Western blot analysis of V3S-0622-YC792 was performed by loading Recombinant Mouse ANGPTL4 Protein.

Antigen: Recombinant Mouse ANGPTL4 Protein
V3S-0622-YC792 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

Figure 3 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in WB

ELISA

Figure 4 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in ELISA

ELISA analysis of V3S-0622-YC792 was performed by coating with Recombinant Mouse ANGPTL4 Protein.

Figure 4 Anti-ANGPTL4 Monoclonal Antibody (V3S-0622-YC792) in ELISA

Expression Species	HEK293F or CHO
Conjugation	None
Purity	>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Protein A affinity purified
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Application	ELISA; WB; FuncS
Application Notes	The antibody is recommended for detection of ANGPTL4 by FuncS assay.

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Target	ANGPTL4
Alternative Name	ANGPTL4; angiopoietin-like 4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL
Research Area	Cell Biology

Anti-ANGPTL4 Neutralizing Antibody (V3S-0622-YC792) (CAT#: V3S-0622-YC792)

Summary

Property

Applications

Protocols

Target

Tested Data

Anti-ANGPTL4 Neutralizing Antibody (V3S-0622-YC792) (CAT#: V3S-0622-YC792)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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