| Sub Cat | Epitope | Affinity | |
|---|---|---|---|
| V3S-1124-YC1578 | E70 of mature huApoC3 is essential for binding of the antibody and do not tolerate exchange by any other amino acids. | 0.156 nM | |
| V3S-0522-YC2929 | T44 and S49 of mature huApoC3 are essential for binding of the antibody and do not tolerate exchange by any other amino acids without a complete or nearly complete loss in binding. | 0.684 nM | |
| V3S-0522-YC2927 | W65 and D66 of mature huApoC3 are essential for binding of the antibody and do not tolerate exchange by any other amino acids. | 1.68 nM |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Apolipoprotein C3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Epitope | E70 of mature huApoC3 is essential for binding of the antibody and do not tolerate exchange by any other amino acids. |
| Affinity | KD = 0.156 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of ApoC3 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
