| Description | This antibody is a human neutralizing antibody specific for ARG2 (human Arginase 2), termed C0020187. It binds to the "sides" of the ARG2 trefoil and shows inhibition to human ARG2. This product has been determined by Enzyme-linked immunosorbent assay, Enzyme inhibition assay, Bio-layer interferometry and Inhibition assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | It binds to ARG2 close to the active site. |
| Neutralization Mechanism | Allosteric mechanism of action: Clone C0020187 binding induces the major conformational changes in ARG2 (aa 34-40, aa 71-88, and aa 150-159) |
| IC50 | 890 nM |
| Affinity | 10 nM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Inhib |
| Application Notes | This antibody was evaluated by ELISA, Bio-layer interferometry (BLI) and Inhibition assay. Test antibodies were preincubated for 2 h at room temperature with 0.4 or 1 μg/mL human ARG2 for inhibition assay. For BLI, antibodies format were titrated as analyte, with an association and dissociation time of 300 s and 600 s, respectively. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
