Anti-B. anthracis PA Neutralizing Antibody (V3S-1022-YC6014) (CAT#: V3S-1022-YC6014)

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Datasheet

MSDS

COA

Sub Cat Host Species Epitope Isotype Application  
V3S-1124-YC77 Human IgG1 lambda FC; IP; ELISA; Neut; FuncS; IF; WB Inquiry
V3S-0522-YC4584 Mouse Located in the sequence: VHASFFDIG. IgG1 Block; ELISA Inquiry
V3S-1022-YC3319 Cynomolgus Located in the sequence: PLYISNPNY. IgG ELISA; Neut Inquiry
V3S-1022-YC3320 Human Located in the sequence: ETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG. IgG ELISA; Neut Inquiry
V3S-1022-YC3323 Mouse Located in the sequence: STSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFF. IgG1 Neut; WB Inquiry
V3S-1022-YC3328 Mouse Located in the sequence: IKLNAKMNILIRDKRFHYDRN. IgG1 WB; Neut; FC Inquiry
V3S-1022-YC5477 Human IgG1 ELISA; Neut Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a Human monoclonal antibody that is capable of recognizing B. anthracis PA. It can be used for B. anthracis PA detection in Flow Cytometry (FC), Immunoprecipitation (IP), Enzyme-Linked Immunosorbent Assay (ELISA), Neutralization Assay (Neut), Functional Assay (FuncS), Immunofluorescence (IF), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Bacillus anthracis
Isotype IgG1 lambda

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC; IP; ELISA; Neut; FuncS; IF; WB

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target B. anthracis PA
Alternative Name PA; anthrax PA; Anthrax Protective Antigen; Anthrax toxins translocating protein; Bacillus anthracis Protective Antigen; PA 83; PA; PA83; Pag; PagA; Protective antigen
Research Area Microbiology
Related Disease Anthrax inhalation
For research use only, not directly for clinical use.
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