| Description | This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for Clostridioides difficile Toxin B, Clostridioides are anaerobic, motile bacteria, ubiquitous in nature, and especially prevalent in soil. It can be used for C. difficile ToxB detection in Western Blot. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Clostridioides difficile |
| Epitope | The epitope is in the C-terminus of C. difficile ToxB. |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C. difficile ToxB |
| Alternative Name | Clostridioides difficile Toxin B; C. difficile ToxB |
| Research Area | Cell Biology |
