Recombinant Anti-C. difficile ToxB (C-terminus) Antibody (V3S-1022-YC38) (CAT#: V3S-1022-YC38)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for Clostridioides difficile Toxin B, Clostridioides are anaerobic, motile bacteria, ubiquitous in nature, and especially prevalent in soil. It can be used for C. difficile ToxB detection in Western Blot. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Clostridioides difficile
Epitope The epitope is in the C-terminus of C. difficile ToxB.
Isotype IgG1

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application WB

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target C. difficile ToxB
Alternative Name Clostridioides difficile Toxin B; C. difficile ToxB
Research Area Immunology
For research use only, not directly for clinical use.
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