| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Complement component 5. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Mouse |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; DB; FC; FuncS; IA; IF; IP; IHC |
| Application Notes | The antibody is recommended for detection of C5 by FC, FuncS, IA, IF, IP, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C5 |
| Alternative Name | C5D; C5a; C5b; ECLZB; CPAMD4 |
| Research Area | Immunology |
Figure 1 Mouse Anti-C5 Monoclonal Antibody Fab Fragment (V3S-0522-YC501) in ELISA
ELISA analysis of V3S-0522-YC501 was performed by coating with mouse complement C5 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0522-YC501. The HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 1 Mouse Anti-C5 Monoclonal Antibody Fab Fragment (V3S-0522-YC501) in ELISA
Figure 2 Mouse Anti-C5 Monoclonal Antibody Fab Fragment (V3S-0522-YC501) in DB
Dot Blot analysis of V3S-0522-YC501 was performed by coating with mouse complement C5 protein (His tag).
The incubation concentration of V3S-0522-YC501 is 2 μg/mL.
HRP conjugated anti-mouse IgG (H+L) as a secondary antibody (1: 3000)
Figure 2 Mouse Anti-C5 Monoclonal Antibody Fab Fragment (V3S-0522-YC501) in DB
