Recombinant Anti-CA9 Antibody (V3S-0522-YC8225) (CAT#: V3S-0522-YC8225)

Figure 1 Anti-CA9 Monoclonal Antibody (V3S-0522-YC8225) in WB.

Western blot analysis of V3S-0522-YC8225 was performed by loading Human CA9 Protein (His Tag).

Figure 2 Anti-CA9 Monoclonal Antibody (V3S-0522-YC8225) in ELISA.

ELISA analysis of V3S-0522-YC8225 was performed by coating with Human CA9 Protein (His Tag).

Figure 3 Anti-CA9 Monoclonal Antibody (V3S-0522-YC8225) in Dot Blot.

Dot Blot analysis of V3S-0522-YC8225 was performed by coating with Human CA9 Protein (His Tag).

Figure 4 Anti-CA9 Monoclonal Antibody (V3S-0522-YC8225) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC8225 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 5 Anti-CA9 Monoclonal Antibody (V3S-0522-YC8225) in SEC-HPLC

The purity of V3S-0522-YC8225 was greater than 97% as determined by HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl