| Description | The antibody C775 is a mouse anti-human MCP-1 mAb that has been developed through the immunization of a Balb/c mouse with DNA encoding the human MCP-1 polypeptide. It is highly specific for human MCP-1 and not for other similar polypeptides like MCP-2, MCP-3, MCP-4, or eotaxin. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Cynomolgus |
| Immunogen | DNA encoding the human MCP-1 polypeptide. |
| IC50 | 7.1 ug/mL |
| Affinity | KD = 1 nM |
| Isotype | Mouse IgG1, kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Inhib |
| Application Notes | The antibody inhibits the chemotactic effect of MCP-1 on THP-1 murine monocyte cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CCL2 |
| Alternative Name | C-C Motif Chemokine Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE; Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je); Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Monocyte Chemoattractant Protein-1; Chemokine (C-C Motif) Ligand 2; Monocyte Chemotactic Protein 1; Small-Inducible Cytokine A2; SCYA2; MCP-1 |
| Related pathway | MIF Mediated Glucocorticoid Regulation and TGF-Beta Pathway |
| Research Area | Cancer Research |
| Related Disease | Inflammatory disease |
