| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Chemokine (C-C motif) receptor 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Full-length human CCR4 (hCCR4) expressing cells |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FC |
| Application Notes | The antibody is recommended for detection of CCR4 by ELISA, WB, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CCR4 |
| Alternative Name | CCR4; chemokine (C-C motif) receptor 4; CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099; C-C chemokine receptor type 4; CCR-4; C-C CKR-4; chemokine (C-C) receptor 4 |
| Gene ID | 1233 |
| UniProt | P51679 |
| Research Area | Immunology; Cardiovascular |
| Related Disease | Primary cutaneous CD30+ lymphoproliferative disorders |
Figure 1 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC6488 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 200 kDa.
Figure 1 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in SDS-PAGE
Figure 2 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in SEC-HPLC
The purity of V3S-0522-YC6488 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 30 µl
Figure 2 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in SEC-HPLC
Figure 3 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in ELISA
ELISA analysis of V3S-0522-YC6488 was performed by coating with CCR4 antigen. Then blocked with BSA, and incubated with Anti-CCR4 Antibody (V3S-0522-YC6488) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-CCR4 Monoclonal Antibody (V3S-0522-YC6488) in ELISA
