Anti-CD2 Neutralizing Antibody (V3S-0622-YC1236) (CAT#: V3S-0622-YC1236)

Figure 1 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC1236 in non-reduced (line 1, 1 μg) and reduced (line 2, 0.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SEC-HPLC

The purity of V3S-0622-YC1236 was greater than 95% as determined by HPLC.

Figure 3 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in WB

Western blot analysis of V3S-0622-YC1236 was performed by loading Recombinant Human CD2 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC1236 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Non-Reduced Antigen (1 μg)
Lane 2: Reduced Antigen (1 μg)

Figure 4 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in ELISA

ELISA analysis of V3S-0622-YC1236 was performed by coating with Recombinant Human CD2 Protein (His Tag). Then blocked with BSA and incubated with Anti-Human CD2 Antibody (V3S-0622-YC1236). The HRP-conjugated goat anti-Human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.