| Description | This product is a human monoclonal antibody that reacts with CD2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human CD2 recombinant protein |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; IP; FC; FuncS; Neut; IF; ICC |
| Application Notes | The antibody is recommended for detection of CD2 by ELISA, IP, FC, FuncS, Neut, IF, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD2 |
| Alternative Name | CD2; CD2 molecule; CD2 antigen (p50), sheep red blood cell receptor, SRBC; T-cell surface antigen CD2; LFA-3 receptor; rosette receptor; erythrocyte receptor; lymphocyte-function antigen-2; T-cell surface antigen T11/Leu-5; CD2 ant |
| Gene ID | 914 |
| UniProt | P06729 |
| Research Area | Immunology |
Figure 1 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC1236 in non-reduced (line 1, 1 μg) and reduced (line 2, 0.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SDS-PAGE
Figure 2 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SEC-HPLC
The purity of V3S-0622-YC1236 was greater than 95% as determined by HPLC.
Figure 2 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in SEC-HPLC
Figure 3 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in WB
Western blot analysis of V3S-0622-YC1236 was performed by loading Recombinant Human CD2 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC1236 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Non-Reduced Antigen (1 μg)
Lane 2: Reduced Antigen (1 μg)
Figure 3 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in WB
Figure 4 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in ELISA
ELISA analysis of V3S-0622-YC1236 was performed by coating with Recombinant Human CD2 Protein (His Tag). Then blocked with BSA and incubated with Anti-Human CD2 Antibody (V3S-0622-YC1236). The HRP-conjugated goat anti-Human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-CD2 Monoclonal Antibody (V3S-0622-YC1236) in ELISA
