| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for CD276 Molecule. It can be used for CD276 Molecule detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Immunohistochemistry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Cynomolgus |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; DB; ELISA; FC; IHC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in ELISA
ELISA analysis of V3S-1022-YC2509 was performed by coating with Human CD276 Protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC2509. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in ELISA
Figure 2 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in WB
Western blot analysis of V3S-1022-YC2509 was performed by loading Human CD276 Protein (His Tag) in reduced condition (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2509 (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 2 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in WB
Figure 3 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in DB
Dot Blot analysis of V3S-1022-YC2509 was performed by coating with Human CD276 Protein (His Tag).
V3S-1022-YC2509 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
Figure 3 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in DB
Figure 4 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in SEC-HPLC
The purity of V3S-1022-YC2509 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 4 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in SEC-HPLC
Figure 5 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in SDS-PAGE
SDS-PAGE analysis of V3S-1022-YC2509 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 5 Anti-CD276 Monoclonal Antibody (V3S-1022-YC2509) in SDS-PAGE
