| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize CD28 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG4 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC |
| Application Notes | The antibody is recommended for detection of CD28 by FC assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC5196 in reduced (lane 2) and non-reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue.
Figure 1 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in SDS-PAGE
Figure 2 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in WB
Western blot analysis of V3S-0522-YC5196 was performed by loading 0.2 µg onto a 12% Tris-HCl polyacrylamide gel in reduced (lane 2) and 0.2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) conditions.
Figure 2 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in WB
Figure 3 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in SEC-HPLC
The purity of V3S-0522-YC5196 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in SEC-HPLC
Figure 4 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in ELISA
ELISA analysis of V3S-0522-YC5196 was performed by coating with Recombinant Human CD28 Protein (11524-HCCH). Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (V3S-0522-YC5196). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-CD28 Monoclonal Antibody (V3S-0522-YC5196) in ELISA
