| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing CD36 molecule. It can be used for Cd36 detection in Enzyme-Linked Immunosorbent Assay (ELISA), Immunoprecipitation (IP), Flow Cytometry (FC), Immunohistochemistry (IHC), Neutralization Assay (Neut). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Rat |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Protein G purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; ELISA; IP; FC; IHC; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Rat Anti-CD38 Monoclonal Antibody (V3S-0822-YC711) in ELISA
ELISA analysis of V3S-0822-YC711 was performed by coating with mouse CD38 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0822-YC711.
The HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 1 Rat Anti-CD38 Monoclonal Antibody (V3S-0822-YC711) in ELISA
Figure 2 Rat Anti-CD38 Monoclonal Antibody (V3S-0822-YC711) in DB
Dot Blot analysis of V3S-0822-YC711 was performed by coating with mouse CD38 protein (His tag).
Incubation concentration of V3S-0822-YC711 is 2 μg/mL.
HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 2 Rat Anti-CD38 Monoclonal Antibody (V3S-0822-YC711) in DB
