| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize CD38 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; ELISA; IHC; FC; IP; IF; FuncS |
| Application Notes | The antibody is recommended for detection of CD38 by ELISA, IHC, FC, IP, IF, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD38 |
| Alternative Name | CD38 Molecule; ADP-Ribosyl Cyclase 1; 2-Phospho-Cyclic-ADP-Ribose Transferase; Cyclic ADP-Ribose Hydrolase 1; 2-Phospho-ADP-Ribosyl Cyclase; NAD(+) Nucleosidase; CD38 Antigen (P45); ADPRC 1; 2-Phospho-ADP-Ribosyl Cyclase/2-Phospho-Cyclic-ADP-Ribose Transferase; Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase; Cluster Of Differentiation 38; CADPr Hydrolase 1; CD38 Antigen; EC 2.4.99.20; EC 3.2.2.6; ADPRC1; T10 |
| Gene ID | 952 |
| UniProt | P28907 |
| Research Area | Immunology |
| Related Disease | Multiple myeloma |
Figure 1 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC1458 in reduced (left) and non-reduced (right) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in SDS-PAGE
Figure 2 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in ELISA
ELISA analysis of V3S-0522-YC1458 was performed by coating with CD38 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 2 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in ELISA
Figure 3 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in DB
Dot Blot analysis of V3S-0522-YC1458 was performed by coating with CD38 Protein, Human, Recombinant (His Tag).
V3S-0522-YC1458 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 3 Anti-CD38 Monoclonal Antibody (V3S-0522-YC1458) in DB
