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Figure 1 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in DB -
Figure 2 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in WB
| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize CD3E. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; FC; IP; ELISA; Neut; FuncS; IF; WB |
| Application Notes | The antibody is recommended for detection of CD3E by FC, IP, ELISA, Neut, FuncS, IF, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in DB
Dot Blot analysis of V3S-0622-YC1300 was performed by coating with human CD3 protein (His tag).
V3S-0622-YC1300 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000)
Figure 1 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in DB
Figure 2 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in WB
Western blot analysis of V3S-0622-YC1300 was performed by loading human CD3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC1300 and HRP goat anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (1.5 μg)
Figure 2 Anti-Human CD3 Monoclonal Antibody (V3S-0622-YC1300) in WB
