| Description | This product is a human monoclonal antibody that reacts with CD4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; FuncS; IF; Neut; ELISA; FC; IP; ICC |
| Application Notes | The antibody is recommended for detection of CD4 by FuncS, IF, Neut, ELISA, FC, IP, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD4 |
| Alternative Name | CD4; CD4 molecule; CD4 antigen (p55), T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut; |
| Gene ID | 920 |
| UniProt | P01730 |
| Related pathway | N-RAS regulation pathway; PEDF Induced Signaling |
| Research Area | Immunology; Stem Cell |
Figure 1 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in SEC-HPLC
The purity of V3S-0622-YC4330 was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 1 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in SEC-HPLC
Figure 2 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in WB
Western blot analysis of V3S-0622-YC4330 was performed by loading Recombinant Human CD4 Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4330 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in WB
Figure 3 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in ELISA
ELISA analysis of V3S-0622-YC4330 was performed by coating with Recombinant Human CD4 Protein. Then blocked with BSA, and incubated with Anti-Human CD4 Antibody (V3S-0622-YC4330). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-CD4 Monoclonal Antibody (V3S-0622-YC4330) in ELISA
