Anti-CD4 (ECD domain) Neutralizing Antibody (V3S-0622-YC4333) (CAT#: V3S-0622-YC4333)

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  • fig1
    Figure 1 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in WB
  • fig1
    Figure 2 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in DB
  • fig1
    Figure 3 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with CD4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Recombinant fragment corresponding to the external domain.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application DB; WB; ELISA; FC; IP; FuncS; IF; Neut
Application Notes The antibody is recommended for detection of CD4 by WB, ELISA, FC, IP, FuncS, IF, Neut assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD4
Alternative Name CD4; CD4 molecule; CD4 antigen (p55), T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut;
Gene ID 920
UniProt P01730
Related pathway N-RAS regulation pathway; PEDF Induced Signaling
Research Area Immunology; Stem Cell

Tested Data

WB

Figure 1 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in WB

Western blot analysis of V3S-0622-YC4333 was performed by loading Recombinant Human CD4 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4333 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 1 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in WB

DB

Figure 2 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in DB

Dot Blot analysis of V3S-0622-YC4333 was performed by coating with Recombinant Human CD4 Protein.

V3S-0622-YC4333 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 2 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in DB

ELISA

Figure 3 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in ELISA

ELISA analysis of V3S-0622-YC4333 was performed by coating with Recombinant Human CD4 Protein. Then blocked with BSA, and incubated with Anti-Human CD4 Antibody (V3S-0622-YC4333). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-Human CD4 Monoclonal Antibody (V3S-0622-YC4333) in ELISA

For research use only, not directly for clinical use.
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