Recombinant Anti-CD5 Antibody (V3S-0522-YC5394) (CAT#: V3S-0522-YC5394)

Figure 1 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS

Flow cytometry analysis of hPBMC cells incubated with Cat# V3S-0522-YC5394. hPBMC cells were stained with V3S-0522-YC5394 (blue histogram) or isotype control antibody (yellow histogram), followed by FITC-conjugated goat anti-Mouse IgG secondary antibody. The used concentration of V3S-0522-YC5394 was 5 µg per 5 x 10⁶ cells.

Figure 2 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS

Flow cytometry analysis of hPBMC cells incubated with Cat# V3S-0522-YC5394. hPBMC cells were stained with V3S-0522-YC5394 (blue histogram) and the FITC-conjugated goat anti-Mouse IgG was as secondary antibody. The scatter diagram was shown.

Figure 3 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in ELISA

ELISA analysis of V3S-0522-YC5394 was performed by coating with human CD5 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0522-YC5394. The HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.

Figure 4 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in WB

Western blot analysis of V3S-0522-YC5394 was performed by loading human CD5 protein, His tag.
The incubation concentration of V3S-0522-YC5394 is 2 ng/μL.
The secondary antibody: HRP-Goat anti-mouse IgG (H+L).

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)

Figure 5 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in DB

Dot Blot analysis of V3S-0522-YC5394 was performed by coating with human CD5 protein (His tag).
The incubation concentration of V3S-0522-YC5394 is 2 μg/mL.
HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.