| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize CD5 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Cynomolgus |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FC; SPR |
| Application Notes | The antibody is recommended for detection of CD5 by FC, SPR assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS
Flow cytometry analysis of hPBMC cells incubated with Cat# V3S-0522-YC5394. hPBMC cells were stained with V3S-0522-YC5394 (blue histogram) or isotype control antibody (yellow histogram), followed by FITC-conjugated goat anti-Mouse IgG secondary antibody. The used concentration of V3S-0522-YC5394 was 5 µg per 5 x 106 cells.
Figure 1 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS
Figure 2 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS
Flow cytometry analysis of hPBMC cells incubated with Cat# V3S-0522-YC5394. hPBMC cells were stained with V3S-0522-YC5394 (blue histogram) and the FITC-conjugated goat anti-Mouse IgG was as secondary antibody. The scatter diagram was shown.
Figure 2 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in FACS
Figure 3 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in ELISA
ELISA analysis of V3S-0522-YC5394 was performed by coating with human CD5 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0522-YC5394. The HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 3 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in ELISA
Figure 4 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in WB
Western blot analysis of V3S-0522-YC5394 was performed by loading human CD5 protein, His tag.
The incubation concentration of V3S-0522-YC5394 is 2 ng/μL.
The secondary antibody: HRP-Goat anti-mouse IgG (H+L).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 4 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in WB
Figure 5 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in DB
Dot Blot analysis of V3S-0522-YC5394 was performed by coating with human CD5 protein (His tag).
The incubation concentration of V3S-0522-YC5394 is 2 μg/mL.
HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 5 Anti-Human CD5 Monoclonal Antibody (V3S-0522-YC5394) in DB
