| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human CD70. It can be used for CD70 detection in Enzyme-Linked Immunosorbent Assay. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-CD70 Monoclonal Antibody (V3S-0822-YC3129) in ELISA
ELISA analysis of V3S-0822-YC3129 was performed by coating with Recombinant Human CD70 Protein (His Tag). Then blocked with BSA and incubated with Anti-Human CD70 Antibody (V3S-0822-YC3129). The HRP-conjugated goat anti-Human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-CD70 Monoclonal Antibody (V3S-0822-YC3129) in ELISA
Figure 2 Anti-CD70 Monoclonal Antibody (V3S-0822-YC3129) in SEC-HPLC
The purity of V3S-0822-YC3129 was greater than 98% as determined by HPLC.
Figure 2 Anti-CD70 Monoclonal Antibody (V3S-0822-YC3129) in SEC-HPLC
