| Description | This product is a human monoclonal antibody that reacts with CD70. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; IP; FuncS; IF; Neut; ICC |
| Application Notes | The antibody is recommended for detection of CD70 by ELISA, FC, IP, FuncS, IF, Neut, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD70 |
| Alternative Name | CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor |
| Gene ID | 970 |
| UniProt | P32970 |
| Related pathway | PEDF Induced Signaling and Cytokine Signaling in Immune system |
| Research Area | Immunology; Cancer Research |
Figure 1 Anti-Human CD70 Monoclonal Antibody (V3S-0622-YC4344) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC4344 in non-reduced (Lane 3, 1 μg) and β-mercaptoethanol reduced (Lane 4, 1 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa. And non-reduced protein migrates as about 180 kDa.
Figure 1 Anti-Human CD70 Monoclonal Antibody (V3S-0622-YC4344) in SDS-PAGE
Figure 2 Anti-Human CD70 Monoclonal Antibody (V3S-0622-YC4344) in ELISA
ELISA analysis of V3S-0622-YC4344 was performed by coating with Recombinant Human CD70, His tag. Then blocked with BSA, and incubated V3S-0622-YC4344. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Human CD70 Monoclonal Antibody (V3S-0622-YC4344) in ELISA
