| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Cadherin 17. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of CDH17 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CDH17 |
| Alternative Name | Cadherin 17; Intestinal Peptide-Associated Transporter HPT-1; Cadherin 17, LI Cadherin (Liver-Intestine); Liver-Intestine Cadherin; Human Intestinal Peptide-Associated Transporter HPT-1; Human Peptide Transporter 1; HPT-1 Cadherin; Cadherin-16; |
| Gene ID | 1015 |
| UniProt | Q12864 |
| Research Area | Signal Pathway |
Figure 1 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in Dot Blot.
Dot blot analysis of V3S-0522-YC6880 was performed by coating with human CDH17 protein (His tag).
Figure 1 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in Dot Blot.
Figure 2 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in Western Blot.
Western blot analysis of V3S-0522-YC6880 was performed by loading human CDH17 protein, His tag.
Figure 2 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in Western Blot.
Figure 3 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in ELISA.
ELISA analysis of V3S-0522-YC6880 was performed by coating with human CDH17 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC6880. The HRP conjugated anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human CDH17 Recombinant Antibody (V3S-0522-YC6880) in ELISA.
