| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Carcinoembyronic antigen. It can be used for Carcinoembyronic antigen detection in Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Affinity | The affinity is in nanomolar range, about 2.0 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CEA |
| Alternative Name | Carcinoembryonic antigen (CEA); CEACAM1 |
| Research Area | Cell Biology |
| Related Disease | Cancer |
Figure 1 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in SDS-PAGE
SDS-PAGE analysis of V3S-1022-YC3244 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 1 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in SDS-PAGE
Figure 2 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in WB
Western blot analysis of V3S-1022-YC3244 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Mouse IgG HRP secondary antibody at a dilution of 1: 8,000 for 75 minutes. Chemiluminescent detection was performed.
Figure 2 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in WB
Figure 3 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in ELISA
ELISA analysis of V3S-1022-YC3244 was performed by coating with human CEA (11077-H08H, 1 µg/mL). Then blocked with BSA, and incubated with Anti-CEA Monoclonal Antibody (V3S-1022-YC3244). The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm. The ELISA curve is shown on the left and the effective dose (ED50) is about 156.12 ng/mL.
Figure 3 Anti-CEA Monoclonal Antibody (V3S-1022-YC3244) in ELISA
