Recombinant Anti-COL7A1 Antibody (V3S-1022-YC2831) (CAT#: V3S-1022-YC2831)

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Datasheet

MSDS

COA

Sub Cat Isotype  
V3S-1124-YC2010 IgA1 Inquiry
V3S-1022-YC2832 IgA2 Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Collagen type VII alpha 1 chain. It can be used for Collagen type VII alpha 1 chain detection in Western Blot, Enzyme-Linked Immunosorbent Assay and Immunofluorescence. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgA1

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application WB; ELISA; IF

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target COL7A1
Alternative Name EBD1; EBR1; EBDCT; NDNC8
Gene ID 1294
UniProt Q02388
Research Area Cell Biology
Related Disease Epidermolysis bullosa acquisita (EBA)
For research use only, not directly for clinical use.
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