| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Dengue virus Envelope (E) Protein Domain III. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Dengue Virus (DENV) |
| Immunogen | DENV Dengue virus E-DIII |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA |
| Application Notes | The antibody is recommended for detection of DENV E-DIII by WB, ELISA assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DENV E-DIII |
| Alternative Name | Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Anti-DENV E-DIII Monoclonal Antibody (V3S-0522-YC7089) in ELISA
ELISA analysis of V3S-0522-YC7089 was performed by coating with Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
The secondary antibody is HRP-goat anti-mouse IgG (H+L).
Figure 1 Anti-DENV E-DIII Monoclonal Antibody (V3S-0522-YC7089) in ELISA
Figure 2 Anti-DENV E-DIII Monoclonal Antibody (V3S-0522-YC7089) in WB
Western blot analysis of V3S-0522-YC7089 was performed by loading Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
The incubation concentration of V3S-0522-YC7089 is 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG (H+L).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 2 Anti-DENV E-DIII Monoclonal Antibody (V3S-0522-YC7089) in WB
