| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Dickkopf WNT signaling pathway inhibitor 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Recombinant FLAG-tagged human DKK1 (FLAG-DKK1) and recombinant Fc-tagged human DKK1 (DKK1-fc) |
| Epitope | Located in amino acids 98 to 104 and a region from amino acids 107-121 and 127-140. |
| Affinity | KD = 3 pM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Block; FuncS |
| Application Notes | The antibody is recommended for detection of DKK1 by ELISA, Block, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DKK1 |
| Alternative Name | Dickkopf WNT Signaling Pathway Inhibitor 1; HDkk-1; DKK-1; SK; Dickkopf (Xenopus Laevis) Homolog 1; Dickkopf 1 Homolog (Xenopus Laevis); Dickkopf Related Protein-1; Dickkopf-Like Protein 1; Dickkopf 1 Homolog; Dickkopf-1 Like; Dickkopf-1 |
| Gene ID | 22943 |
| UniProt | O94907 |
| Research Area | Immunology; Cardiovascular |
| Related Disease | Cancer, Diabetes |
