| Description | This product is a human monoclonal antibody that reacts with epidermal growth factor (EGFR). The antibody AbA has improved binding affinity for EGFR. AbA binds to EGFR protein in an in vitro keratinocyte binding assay. Further, antibody AbA is able to inhibit or decrease tumor growth in a xenograft H292 cell assay and was comparable at inhibiting cell growth in an in vivo assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | The epitope is located in the CR1 domain (domain II), aa 287-302. |
| Affinity | 220 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; Inhib; FuncS |
| Application Notes | The antibody is recommended for detection of EGFR by ELISA, FC, Inhib, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EGFR |
| Alternative Name | ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1 |
| Gene ID | 1956 |
| UniProt | P00533 |
| Research Area | Signal Pathway |
