| Description | This product is a human monoclonal antibody that binds to the class III variant of EGFR (EGFRvIII) with high affinity. The antibody showed selectivity for EGFRvIII and did not bind wild-type EGFR. It has proved that the EGFRvIII antibody H1H1863N2 has specificity for EGFRvIII-expressing cell lines and specific cell killing ability when conjugated to the DM1 toxin. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | The extracellular domain of EGFRvIII. |
| Epitope | The epitope is located in the extracellular domain. |
| Affinity | 0.21 nM |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib; FuncS |
| Application Notes | The antibody is recommended for detection of EGFRvIII by ELISA, Inhib, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EGFRvIII |
| Alternative Name | Class III variant of the EGFR |
| Research Area | Cancer Research |
