| Description | This product is a human monoclonal antibody that reacts with ERBB3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | HER3 ECD protein |
| Epitope | In the extracellular domain |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; IP; IF; FuncS; FC; Neut; ELISA; ICC |
| Application Notes | The antibody is recommended for detection of ERBB3 by IP, IF, FuncS, FC, Neut, ELISA, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ERBB3 |
| Alternative Name | ERBB3; erb-b2 receptor tyrosine kinase 3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; receptor tyrosine-protein kinase erbB-3; proto-oncogene-like protein c-ErbB-3; human epidermal growth factor receptor |
| Gene ID | 2065 |
| UniProt | P21860 |
| Related pathway | PI3K/Akt pathway |
| Research Area | Signal Pathway |
Figure 1 Human Anti-ERBB3 Monoclonal Antibody (V3S-0622-YC4450) in WB
Western blot analysis of V3S-0622-YC4450 was performed by loading ERBB3 Protein, Human.
The secondary antibody: HRP Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.6 μg)
Lane 2: Reducing Antigen (1.0 μg)
Lane 3: Reducing Antigen (1.5 μg)
Figure 1 Human Anti-ERBB3 Monoclonal Antibody (V3S-0622-YC4450) in WB
Figure 2 Human Anti-ERBB3 Monoclonal Antibody (V3S-0622-YC4450) in ELISA
ELISA analysis of V3S-0622-YC4450 was performed by coating with ERBB3 Protein, Human.
The secondary antibody: HRP Anti-Human IgG (H+L)
Figure 2 Human Anti-ERBB3 Monoclonal Antibody (V3S-0622-YC4450) in ELISA
