| Description | This product is a human neutralizing antibody clone 38-3-11A binding EV71 capsid. It neutralized virus at picomolar level and fully protected mice from a lethal EV71 infection at a prophylactic dose of 0.4 mg/kg. In pre- and post-attachment neutralization assays, neutralizing antibodies 38-3-11A has potent inhibitory activity. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | EV71 |
| Epitope | 38-3-11A recognizes a threefold proximal epitope composed of VP2 BC and HI loops from one protomer and VP3 BC and HI loops of another protomer from a neighbouring pentamer. |
| EC50 | 0.117 μg/mL |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; IP; WB; Neut |
| Application Notes | The antibody was detected by ELISA, Flow cytometry (FC) and Neutralization assay. ELISA: The antibody-containing cell culture supernatants or purified antibody was applied and bound virus_x0002_specific antibody was detected with HRP-conjugated rabbit anti-human IgG secondary antibody. Flow cytometry (FC): 5 μg/mL Neutralization assay (Neut): Serially diluted antibody in virus dilution medium was incubated with 100 TCID50 of virus preparation at 37°C for 2 h. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EV71 VP |
| Alternative Name | Enterovirus 71 capsid protein |
| Research Area | Infectious Disease |
| Related Disease | Meningitis, Encephalitis and Acute flaccid myelitis |
