Recombinant Anti-Extended Type I Chain GSL Antibody (V3S-0822-YC3002) (CAT#: V3S-0822-YC3002)

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Datasheet

MSDS

COA

Sub Cat Application  
V3S-1124-YC1856 ELISA; Cyt Inquiry
V3S-0822-YC3003 ELISA; FC; Cyt Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing Extended Type I Chain Glycosphingolipid. It can be used for Extended Type I Chain GSL detection in Enzyme-Linked Immunosorbent Assay (ELISA), Cytotoxicity Assay (Cyt). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human
Epitope The epitope of mAb was specified as extended Type I chain Leb-Lea based on the results of HPTLC immunostaining and ELISA.
Isotype IgG

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A or G purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; Cyt

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target Extended Type I Chain GSL
Alternative Name Extended Type I Chain Glycosphingolipid (GSL)
Research Area Cellular Marker
For research use only, not directly for clinical use.
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