Recombinant Anti-F2RL1 Antibody (V3S-0522-YC6639) (CAT#: V3S-0522-YC6639)

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Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Coagulation factor II (thrombin) receptor-like 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Either soluble PAR-2, PAR-2 expressing cells or PAR-2/Fc protein
Affinity KD = 63 pM, determined by surface plasmon resonance.
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application ELISA; WB; FuncS
Application Notes The antibody is recommended for detection of F2RL1 by ELISA, WB, FuncS assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target F2RL1
Alternative Name F2RL1; coagulation factor II (thrombin) receptor-like 1; PAR2; GPR11; proteinase-activated receptor 2; thrombin receptor-like 1; G-protein coupled receptor 11; protease-activated receptor 2; coagulation factor II receptor-like 1
Gene ID 2150
UniProt P55085
Research Area Immunology
Related Disease PAR-2-related disorder
For research use only, not directly for clinical use.
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