Anti-F3 Neutralizing Antibody (V3S-0522-YC8247) (CAT#: V3S-0522-YC8247)

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Datasheet

MSDS

COA

Sub Cat Epitope Isotype Application  
V3S-1124-YC843 IgG1 kappa Neut Inquiry
V3S-1022-YC3859 The epitope contains residues K154, K181, I184, T186, Y188, Y189, K197, T199, K201, N203, D210, Q222, P226, R232, K233, D236. IgG ELISA; Inhib Inquiry
V3S-0522-YC919 In the extracellular domain IgG1 kappa ELISA; FuncS Inquiry
V3S-0522-YC1288 IgG ELISA; FC Inquiry
V3S-0522-YC6450 IgG ELISA; WB Inquiry
V3S-0522-YC8202 IgG1 kappa WB; ELISA; IF; FuncS Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Coagulation factor III (thromboplastin, tissue factor). The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Human coagulation factor III (thromboplastin, tissue factor)
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application Neut
Application Notes The antibody is recommended for detection of F3 by Neut assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target F3
Alternative Name F3; coagulation factor III (thromboplastin, tissue factor); TF; TFA; CD142; tissue factor
Gene ID 2152
UniProt P13726
Research Area Cardiovascular
For research use only, not directly for clinical use.
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