Recombinant Anti-FAP Antibody (V3S-1022-YC2448) (CAT#: V3S-1022-YC2448)

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  • fig1
    Figure 1 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in ELISA
  • fig1
    Figure 2 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in WB
  • fig1
    Figure 3 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Fibroblast Activation Protein Alpha. It can be used for Fibroblast Activation Protein Alpha detection in Western Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen Recombinant human FAP
Affinity The affinity is in nanomolar range, about 1.1 ± 0.4 nM, determined by surface plasmon resonance.
Isotype IgG1

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application DB; WB; ELISA; FC; IHC; IP

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target FAP
Alternative Name FAP; fibroblast activation protein, alpha; FAPA; SIMP; DPPIV; prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase;
Gene ID 2191
UniProt B4DLR2
Research Area Cell Biology; Cancer Research
Related Disease Cancer

Tested Data

ELISA

Figure 1 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in ELISA

ELISA analysis of V3S-1022-YC2448 was performed by coating with human FAP protein active dimer (His Tag).
The secondary antibody: HRP-anti-human IgG (H+L)

Figure 1 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in ELISA

WB

Figure 2 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in WB

Western blot analysis of V3S-1022-YC2448 was performed by loading human FAP protein active dimer (His Tag).
The incubation concentration of V3S-1022-YC2448 is 2 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 2 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in WB

DB

Figure 3 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in DB

Dot blot analysis of V3S-1022-YC2448 was performed by loading human FAP protein active dimer (His Tag).
The incubation concentration of V3S-1022-YC2448 is 2 μg/mL.
The secondary antibody: HRP-anti-human IgG (H+L)

Figure 3 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in DB

For research use only, not directly for clinical use.
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