| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Fibroblast Activation Protein Alpha. It can be used for Fibroblast Activation Protein Alpha detection in Western Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Recombinant human FAP |
| Affinity | The affinity is in nanomolar range, about 1.1 ± 0.4 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; FC; IHC; IP |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FAP |
| Alternative Name | FAP; fibroblast activation protein, alpha; FAPA; SIMP; DPPIV; prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase; |
| Gene ID | 2191 |
| UniProt | B4DLR2 |
| Research Area | Cell Biology; Cancer Research |
| Related Disease | Cancer |
Figure 1 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in ELISA
ELISA analysis of V3S-1022-YC2448 was performed by coating with human FAP protein active dimer (His Tag).
The secondary antibody: HRP-anti-human IgG (H+L)
Figure 1 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in ELISA
Figure 2 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in WB
Western blot analysis of V3S-1022-YC2448 was performed by loading human FAP protein active dimer (His Tag).
The incubation concentration of V3S-1022-YC2448 is 2 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in WB
Figure 3 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in DB
Dot blot analysis of V3S-1022-YC2448 was performed by loading human FAP protein active dimer (His Tag).
The incubation concentration of V3S-1022-YC2448 is 2 μg/mL.
The secondary antibody: HRP-anti-human IgG (H+L)
Figure 3 Human Anti-FAP Monoclonal Antibody (V3S-1022-YC2448) in DB
