| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Fibroblast Growth Factor Receptor 2. It can be used for Fibroblast Growth Factor Receptor 2 detection in Enzyme-Linked Immunosorbent Assay, Western Blot and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Recombinant Human FGFR2 protein |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FGFR2 |
| Alternative Name | Fibroblast Growth Factor Receptor 2; Keratinocyte Growth Factor Receptor; Bacteria-Expressed Kinase; EC 2.7.10.1; K-SAM; KGFR; BEK; Protein Tyrosine Kinase, Receptor Like 14; BEK Fibroblast Growth Factor Receptor; Craniofacial Dysostosis 1; Jackson-Weiss Syndrome; Pfeiffer Syndrome; Crouzon Syndrome; CD332 Antigen; EC 2.7.10; FGFR-2; BFR-1; CD332; BBDS; CEK3; ECT1; TK14; TK25; CFD1; KSAM; JWS |
| Gene ID | 2263 |
| UniProt | P21802 |
| Research Area | Cardiovascular; Signal Pathway |
| Related Disease | FGFR2 -related diseases or conditions such as cancer |
Figure 1 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in Western Blot.
Western blot analysis of V3S-1022-YC2610-YJ was performed by loading recombinant human FGFR2 protein (ECD, His Tag).
V3S-1022-YC2610-YJ incubation concentration: 2ng/μL.
The secondary antibody: HRP-goat anti-human IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in Western Blot.
Figure 2 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in ELISA.
ELISA analysis of V3S-1022-YC2610-YJ was performed by coating with recombinant human FGFR2 protein (ECD, His Tag).
The secondary antibody: HRP-goat anti-human IgG (H+L)
Figure 2 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in ELISA.
Figure 3 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in Dot Blot.
Dot blot analysis of V3S-1022-YC2610-YJ was performed by coating with recombinant human FGFR2 protein (ECD, His Tag).
V3S-1022-YC2610-YJ incubation concentration: 2ng/μL.
The secondary antibody: HRP-goat anti-human IgG (H+L)
Figure 3 Anti-FGFR2 Recombinant Antibody (V3S-1022-YC2610) in Dot Blot.
