Recombinant Anti-FGFR3 (domain 2) Antibody (V3S-0522-YC6514) (CAT#: V3S-0522-YC6514)

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  • fig1
    Figure 1 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SDS-PAGE
  • fig1
    Figure 2 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SEC-HPLC
  • fig1
    Figure 3 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Fibroblast growth factor receptor 3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Epitope Located in domain 2 of FGFR3.
Affinity KD = 1.5 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC; FuncS
Application Notes The antibody is recommended for detection of FGFR3 by FC, FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target FGFR3
Alternative Name Fibroblast Growth Factor Receptor 3; EC 2.7.10.1; FGFR-3; JTK4; Fibroblast Growth Factor Receptor 3 Variant 4; Achondroplasia, Thanatophoric Dwarfism; Hydroxyaryl-Protein Kinase; Tyrosine Kinase JTK4; CD333 Antigen; HSFGFR3EX; EC 2.7.10; CD333; CEK2; ACH
Gene ID 2261
UniProt P22607
Research Area Cell Biology; Cardiovascular
Related Disease Inflammatory autoimmune disease, Rheumatoid arthritis

Tested Data

SDS-PAGE

Figure 1 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC6514 in reduced (lane 1) and non-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 30 kDa, respectively.

Figure 1 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SEC-HPLC

The purity of V3S-0522-YC6514 was greater than 95% as determined by HPLC.

Figure 2 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SEC-HPLC

ELISA

Figure 3 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in ELISA

ELISA analysis of V3S-0522-YC6514 was performed by coating with with recombinant human FGFR3 protein (ECD, His Tag).

Figure 3 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in ELISA

For research use only, not directly for clinical use.
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