| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Fibroblast growth factor receptor 3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Epitope | Located in domain 2 of FGFR3. |
| Affinity | KD = 1.5 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; FuncS |
| Application Notes | The antibody is recommended for detection of FGFR3 by FC, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FGFR3 |
| Alternative Name | Fibroblast Growth Factor Receptor 3; EC 2.7.10.1; FGFR-3; JTK4; Fibroblast Growth Factor Receptor 3 Variant 4; Achondroplasia, Thanatophoric Dwarfism; Hydroxyaryl-Protein Kinase; Tyrosine Kinase JTK4; CD333 Antigen; HSFGFR3EX; EC 2.7.10; CD333; CEK2; ACH |
| Gene ID | 2261 |
| UniProt | P22607 |
| Research Area | Cell Biology; Cardiovascular |
| Related Disease | Inflammatory autoimmune disease, Rheumatoid arthritis |
Figure 1 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC6514 in reduced (lane 1) and non-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 30 kDa, respectively.
Figure 1 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SDS-PAGE
Figure 2 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SEC-HPLC
The purity of V3S-0522-YC6514 was greater than 95% as determined by HPLC.
Figure 2 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in SEC-HPLC
Figure 3 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in ELISA
ELISA analysis of V3S-0522-YC6514 was performed by coating with with recombinant human FGFR3 protein (ECD, His Tag).
Figure 3 Anti-FGFR3 Monoclonal Antibody (V3S-0522-YC6514) in ELISA
