| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Folate receptor 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Monkey |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; IHC |
| Application Notes | The antibody is recommended for detection of FOLR1 by ELISA, WB, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FOLR1 |
| Alternative Name | FOLR1; folate receptor 1; FBP; FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18 |
| Gene ID | 2348 |
| UniProt | P15328 |
| Research Area | Signal Pathway |
| Related Disease | Ovarian cancer |
Figure 1 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC1032 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 1 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in SDS-PAGE
Figure 2 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in SEC-HPLC
The purity of V3S-0522-YC1032 was greater than 77% as determined by SEC-HPLC.
Figure 2 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in SEC-HPLC
Figure 3 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in WB
Western blot analysis of V3S-0522-YC1032 was performed by loading Recombinant Human FOLR1 Protein (lane 2, 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC1032 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in WB
Figure 4 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in ELISA
ELISA analysis of V3S-0522-YC1032 was performed by coating with Recombinant Human FOLR1 Protein. Then blocked with BSA, and incubated with Anti-Human FOLR1 Monoclonal Antibody (HuRA15-7CTAcc) (V3S-0522-YC1032). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-FOLR1 Monoclonal Antibody (V3S-0522-YC1032) in ELISA
