| Sub Cat | Epitope | Isotype | Application | Affinity | |
|---|---|---|---|---|---|
| V3S-1124-YC278 | IgG2 | ELISA; WB | |||
| V3S-0622-YC1652 | IgG | ELISA; FC | |||
| V3S-1022-YC3013 | IgG1 kappa | WB; ELISA | 0.099 nM (mFc-rhGDF15) | ||
| V3S-1022-YC3014 | IgG1 kappa | WB; ELISA | 0.014 nM (mFc-rhGDF15) | ||
| V3S-1022-YC3017 | IgG1 kappa | WB; ELISA | 0.16 nM (mFc-rhGDF15) | ||
| V3S-0622-YC186 | It is a conformational or a discontinuous epitope. These peptides are restricted to the positions 40- 55 (EVQVTMCIGACPSQFR) and 94-114 (TDTGVSLQTYDDLLAKDCHCI) in the GDF-15 amino acid sequence. | IgG1 kappa | Inhib; FuncS | 790 pM | |
| V3S-0622-YC187 | It is a conformational or a discontinuous epitope. These peptides are restricted to the positions 40- 55 (EVQVTMCIGACPSQFR) and 94-114 (TDTGVSLQTYDDLLAKDCHCI) in the GDF-15 amino acid sequence. | IgG1 kappa | Inhib; FuncS |
| Description | This product is a human monoclonal antibody that reacts with GDF15. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG2 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB |
| Application Notes | The antibody is recommended for detection of GDF15 by ELISA, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | GDF15 |
| Alternative Name | GDF15; growth differentiation factor 15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1 |
| Gene ID | 9518 |
| UniProt | Q99988 |
| Research Area | Signal Pathway |
