| Description | The antibody has cross-reactive with a range of bacterial species, with the highest binding consistently demonstrated towards E. coli, P. mirabilis, S. marcescens, and S. typhimurium. The mAb displayed a high affinity at anomolar level for Salmonella flagellin. Subsequent experiments confirmed the mAb's inhibitory effect on flagellin activity. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Gram-negative bacteria |
| Cross Reactivity | E. coli, P. mirabilis, S. marcescens, and S. typhimurium |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Block, DB, FC, WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
