| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Influenzavirus A Subtype H5N1 Hemagglutinin. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Influenza A virus (IAV) |
| Immunogen | IAV haemagglutinin |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Neut |
| Application Notes | The antibody is recommended for detection of H5N1 HA by ELISA, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | H5N1 HA |
| Alternative Name | HA; haemagglutinin; HA1; HA2 |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC7700 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in SDS-PAGE.
Figure 2 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in SEC-HPLC.
The purity of V3S-0522-YC7700 was 99.3837% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in SEC-HPLC.
Figure 3 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in ELISA.
ELISA analysis of V3S-0522-YC7700 was performed by coating with Influenza A H5N1 (A/VietNam/1203/2004) Hemagglutinin protein (HA1 Subunit) (His & Fc Tag).
Figure 3 Anti-H5N1 HA Antibody (V3S-0522-YC7700) in ELISA.
