| Description | This product is a human monoclonal antibody that reacts with HAVCR2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human TIM-3 (for panning) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FuncS |
| Application Notes | The antibody is recommended for detection of HAVCR2 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HAVCR2 |
| Alternative Name | T-cell immunoglobulin and mucin domain containing molecule-3; TIM3 |
| Research Area | Cell Biology |
Figure 1 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC2647 in reduced conditions (Left) and non-reduced (Right) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 150-200 kDa.
Figure 1 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SDS-PAGE
Figure 2 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SEC-HPLC
The purity of V3S-0622-YC2647 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SEC-HPLC
Figure 3 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in WB
Western blot analysis of V3S-0622-YC2647 was performed by loading Recombinant Human TIM3, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2647 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in WB
Figure 4 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in ELISA
ELISA analysis of V3S-0622-YC2647 was performed by coating with Recombinant Human TIM3, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (V3S-0622-YC2647). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in ELISA
