Anti-HAVCR2 Neutralizing Antibody (V3S-0622-YC2647) (CAT#: V3S-0622-YC2647)

Figure 1 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC2647 in reduced conditions (Left) and non-reduced (Right) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 150-200 kDa.

Figure 2 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in SEC-HPLC

The purity of V3S-0622-YC2647 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in WB

Western blot analysis of V3S-0622-YC2647 was performed by loading Recombinant Human TIM3, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2647 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 4 Anti-HAVCR2 Monoclonal Antibody (V3S-0622-YC2647) in ELISA

ELISA analysis of V3S-0622-YC2647 was performed by coating with Recombinant Human TIM3, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (V3S-0622-YC2647). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.