| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Hepatitis C virus envelope protein E2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Hepatitis C Virus (HCV) |
| Immunogen | Hepatitis C virus E2 envelope protein |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; IF; FuncS |
| Application Notes | The antibody is recommended for detection of HCV E2 by WB, ELISA, IF, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HCV E2 |
| Alternative Name | E2; HCV; envelope protein; hepatitis C virus |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC7601 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
Figure 1 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in SDS-PAGE.
Figure 2 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in SEC-HPLC.
The purity of V3S-0522-YC7601 was 96.2631% as determined by SEC-HPLC.
Figure 2 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in SEC-HPLC.
Figure 3 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in Western Blot.
Western blot analysis of V3S-0522-YC7601 was performed by loading HCV E2 protein (His Tag) protein, His tag.
Figure 3 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in Western Blot.
Figure 4 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in ELISA.
ELISA analysis of V3S-0522-YC7601 was performed by coating with HCV E2 protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC7601. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-HCV E2 Recombinant Antibody (V3S-0522-YC7601) in ELISA.
