| Description | This product is a human monoclonal antibody that recognizes Env third variable loop (V3)-glycan (Man9-V3) and can be used in BLI. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Immunogen | Synthetic V3-loop glycopeptide |
| Epitope | The epitope contains two major regions of antibody-glycopeptide interactions: (1) the Env 324GDIR327 motif and (2) the N332-Man9. |
| Neutralization Breath | 42% neutralization breadth |
| Affinity | KD = 7 ± 0.4 μM (Man9-V3) KD = 1.2 ± 0.3 μM (gp120) KD = 2.76 nM (wild-type Env) KD = 7.64 nM (R327A mutant form of 92BR SOSIP.664 Env) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; BLI |
| Application Notes | Biolayer interferometry: 0.2 mg/ml |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 Env |
| Alternative Name | Man9-V3 |
| Research Area | Microbiology; Infectious Disease |
