| Description | This product is a human monoclonal antibody that reacts with HIV1 gp41. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human Immunodeficiency Virus (HIV) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; ELISA; FC |
| Application Notes | The antibody is recommended for detection of HIV1 gp41 by ELISA, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Human Anti-HIV-1 gp160 Monoclonal Antibody (V3S-0622-YC3388) in ELISA
ELISA analysis of V3S-0622-YC3388 was performed by coating with recombinant HIV1 gp41 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0622-YC3388. The HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.
Figure 1 Human Anti-HIV-1 gp160 Monoclonal Antibody (V3S-0622-YC3388) in ELISA
Figure 2 Human Anti-HIV-1 gp160 Monoclonal Antibody (V3S-0622-YC3388) in DB
Dot Blot analysis of V3S-0622-YC3388 was performed by coating with recombinant HIV1 gp41 protein (His tag).
The incubation concentration of V3S-0622-YC3388 is 2 μg/mL.
HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.
Figure 2 Human Anti-HIV-1 gp160 Monoclonal Antibody (V3S-0622-YC3388) in DB
